Nazarin mu ya nuna cewa Ganoderma lucidum / reishi / lingzhi yana nuna kaddarorin antitumor akan ƙwayoyin osteosarcoma a cikin vitro.An gano cewa Ganoderma lucidum yana hana haɓakar ƙwayar nono da ƙaura ta hanyar hana siginar Wnt/β-catenin.Yana hana kansar huhu ta hanyar rugujewar mannewa mai hankali da shigar da lalatawar Slug mai matsakaicin MDM2.Ganoderma lucidum yana hana ciwon nono ta hanyar rage tsarin PI3K/AKT/mTOR, Ganoderma lucidum yana taka rawar antitumor a cikin ƙwayoyin cutar sankarar bargo ta hanyar toshe hanyar MAPK.
CCK-8 da ƙididdigar ƙididdiga na mulkin mallaka, don kimanta tasirin Ganoderma lucidum akan layin layin osteosarcoma da haɓakawa, ya nuna cewa Ganoderma lucidum yana hana haɓakar ƙwayoyin MG63 da U2-OS a cikin lokaci-da kuma dogara da hankali, kuma yana rage raguwa. ikon sel don yin mulkin mallaka.
Ganoderma lucidum yana haɓaka maganganun kwayoyin halitta na proapoptotic, kuma bincike na cytometry ya kwarara ya nuna cewa apoptosis na MG63 da U2-OS sun karu bayan jiyya tare da Ganoderma lucidum.Hijira ta salula shine tushen ɗabi'un ɗabi'un halittu, waɗanda suka haɗa da angiogenesis, warkar da rauni, kumburi, da ciwon daji.Ganoderma lucidum yana rage ƙaura da mamayewa na duka layin salula kuma yana hana haɓakawa, ƙaura, da mamayewa, kuma yana haifar da apoptosis na ƙwayoyin osteosarcoma.
Aberrant Wnt / β-catenin siginar yana da alaƙa da haɓakawa, metastasis, da apoptosis na nau'ikan cututtukan daji da yawa, tare da haɓakar siginar Wnt / β-catenin a cikin osteosarcoma.
A cikin wannan binciken, dual-luciferase reporter assays ya nuna cewa Ganoderma lucidum magani ya toshe CHIR-99021- kunna Wnt / β-catenin siginar.An ƙara tabbatar da wannan ta hanyar nunin mu cewa rubutun Wnt da aka yi niyya, kamar LRP5, β-catenin, cyclin D1, da MMP-9, an hana su lokacin da aka bi da ƙwayoyin osteosarcoma tare da Ganoderma lucidum.
Nazarin da suka gabata sun nuna a cikin samfurori na asibiti cewa LRP5 yana haɓakawa a cikin osteosarcoma dangane da nama na al'ada, kuma bayanin LRP5 yana daidaitawa tare da cututtuka na metastatic da marasa lafiya marasa lafiya, yana sa LRP5 ya zama maƙasudin magani na osteosarcoma.
β-catenin kanta shine maɓalli mai mahimmanci a cikin hanyar siginar Wnt / β-catenin, kuma maganganun β-catenin a cikin osteosarcoma yana ƙaruwa sosai.Lokacin da β-catenin ya juya cikin tsakiya daga cytoplasm, yana kunna maganganun kwayoyin halittar da ke cikin ƙasa, wanda ya haɗa da cyclin D1, C-Myc, da MMPs.
Myc yana daya daga cikin manyan proto-oncogenes kuma yana taka muhimmiyar rawa wajen daidaita kunnawa, rubutawa, da kuma hana bayyanar kwayoyin halitta.An ruwaito cewa kashewar C-Myc oncogene yana haifar da tsufa da apoptosis na nau'in ƙwayar ƙwayar cuta da dama, ciki har da osteosarcoma.
Cyclin D1 shine muhimmin mai tsara tsarin zagayowar tantanin halitta na G1 kuma yana haɓaka canjin lokaci na G1/S.Yawan magana na cyclin D1 na iya rage sake zagayowar tantanin halitta kuma yana haɓaka saurin yaduwa tantanin halitta a cikin nau'ikan ƙari daban-daban.
MMP-2 da MMP-9 su ne stromelysins waɗanda ke da ikon lalata abubuwan haɗin matrix na extracellular, muhimmin fasali don angiogenesis na ƙari da mamayewa.
Wannan yana nuna cewa Wnt / β-catenin kwayoyin halitta suna taka muhimmiyar rawa a ci gaban osteosarcoma, kuma toshe waɗannan nodes na siginar na iya samun sakamako mai ban mamaki.
Daga baya, mun gano bayanin mRNA da furotin na Wnt/β-catenin da ke da alaƙa da siginar ƙwayoyin cuta ta PCR da lalatawar yamma.A cikin duka layin salula, Ganoderma lucidum ya hana maganganun waɗannan sunadaran da kwayoyin halitta.Wadannan sakamakon sun kara nuna cewa Ganoderma lucidum ya hana Wnt / β-catenin siginar ta hanyar ƙaddamar da LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, da MMP-9.
E-cadherin shine glycoprotein transmembrane wanda aka bayyana a cikin sel epithelial kuma yana daidaita mannewa tsakanin sel epithelial da ƙwayoyin stromal.Gogewa ko asarar maganganun E-cadherin yana haifar da asara ko raunin mannewa tsakanin ƙwayoyin tumor, yana ba da damar ƙwayoyin tumo su iya motsawa cikin sauƙi, sa'an nan kuma sanya ƙwayar ƙwayar cuta ta shiga, yaduwa, da kuma daidaitawa.A cikin wannan binciken, mun gano cewa Ganoderma lucidum na iya haɓaka E-cadherin, ta haka yana fuskantar phenotype na Wnt/β-catenin-matsakaicin ƙwayoyin osteosarcoma.
A ƙarshe, sakamakonmu yana nuna cewa Ganoderma lucidum yana toshe osteosarcoma Wnt/β-catenin siginar kuma a ƙarshe yana haifar da raguwar ayyukan ƙwayar osteosarcoma.Wadannan binciken sun nuna cewa Ganoderma lucidum na iya zama wakili mai amfani da tasiri don maganin osteosarcoma, samfurori masu dangantaka sun haɗa da.ganoderma lucidum spore oil softgels/reishi spore oil softgels,ganoderma lucidum spore foda/reishi spore foda
Lokacin aikawa: Afrilu-18-2022